At BMCU, we are equipped with a number of instruments to probe biomolecular interactions.
These include optical (surface plasmon resonance) and gravimetric (quartz crystal microbalance with dissipation) biosensing instruments, as well as fully automated isothermal calorimetry. In addition, using our LigandTracer® Green instrument we can do real-time quantification of binding of fluorescently labeled biomolecules or bacterial cells to immobilized cells or receptors. We can assist with measurement of interactions of proteins with other proteins, RNA, lipids carbohydrates or small molecules.
For further information you can contact Johan Olofsson Edlund, or the person responsible for the instrument you are interested in (listed below together with the instrument).
Quartz Crystal Microbalance with Dissipation monitoring (QCM-D)
ImageJohanMore details
Location
The room is: Building 6M, 4th floor, room 6070
6070, 6M, Norrlands universitetssjukhus Nus,
Price
500 SEK/day
Introduction/driver license Cost is 2500 SEK
Sensor discs (Common) 700 Sek/each (*)
*can last between 1-10 measurements depending on running conditions
Contact Person
Hudson Pace
About
Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) is a label free technique that allows for the real-time monitoring of changes in both mass adsorbed to the sensor surface and in the viscoelastic properties of materials adsorbed to the sensor surface. While this instrument can be used for a wide variety of experiments ranging from materials science to health care, the host lab commonly uses it to study how proteins interact with lipid membranes and/or for investigating protein-protein interactions. While SPR instruments (also available at BICU) provide the dry mass of the adsorbed protein, this instrument measures the hydrated mass which when combined with the dissipation monitoring allows, among other, changes in packing density of proteins interacting with the surface to be resolved.
ImageJohan
More details
Location
The room is: Building KBC, 6th floor, room A5 39 07.
Price
1000 SEK/day
Introduction/driver license Cost is 1000 SEK (half a day)
About
Auto-ITC200 is a fully automatec isothermal caliometry system that measures affinity based on heat exchange when two molecules interact. It can be used to determine the dissociation constant, enthalpy, entropy and stoichiometry of interactions. The most common applications are protein-protein and protein-small molecule interactions, but it will be able to measure any type of interaction that gives of a large enough signal. IN comparison with the SPR systems the ITC measures in solution and not bound on surfaces, so while you may need more materal compared to the SPR approach, you will not need to worry about any surface immobilizaton.
ITC200, BioMolecular Characterization Umeå, BICU
ImageRonnie BerntssonMore details
Location
The room is: Building KBC, 6th floor, room A5 39 07
Price
1000 SEK/day
Introduction/driver license Cost is 1000 SEK (half a day)
About
ITC200 is a manual isothermal caliometry system that measures affinity based on heat exchange when two molecules interact. As with the Auto-ITC200, it can be used to determine the dissociation constant, enthalpy, entropy and stoichiometry of interactions.
The most common applications are protein-protein and protein-small molecule interactions, but it will be able to measure any type of interaction that gives of a large enough signal. In comparison with the SPR systems the ITC measures in solution and not bound on surfaces, so while you may need more materal compared to the SPR approach, you will not need to worry about any surface immobilizaton.
The advantage of the manual ITC200 vs the Auto-ITC200 is that it consumes slightly less sample than the automated one. The downside is that you need to perform each run manually.
ImageJohan
More details
Location
The room is: Building KBC, 6th floor, room A6 31 01.
Price
500 SEK/day
Introduction/driver license Cost is 1500 SEK
About
LigandTracer® Green – an instrument for real-time quantification of binding of fluorescently labeled biomolecules or bacterial cells to immobilized cells or receptors.
LigandTracer® Green offers a simple and accurate method for detection and quantification of biomolecular/cells interactions in real‐time using fluorescently labeled biomolecules. Measurements of e.g. on‐off rate, affinity or retention can be made using blue (~488nm) and green (~535nm) fluorophores, e.g. FITC, Alexa Fluor® 488, as labels. A detector for Yellow (590nm) - Red (632nm) for Rhodamine Red and similar fluorophores is also available.